Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 3. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Picogreen assays are compatible with most microplate readers and fluorometers. 2 – 100 ng. PicoGreen dsDNA quantitation reagents are. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. 05 to consider statistical significance. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Catalog Number. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assays are compatible with most microplate readers and fluorometers. Using this data as a starting point, the optimal conditions (e. • Specific for dsDNA in the presence of RNA. Barcoded amplicons were pooled in. 99. For Research Use Only. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Picogreen assays are compatible with most microplate readers and fluorometers. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. PicoGreen dsDNA quantitation reagents are. quant it picogreen dsdna assay kit reagents. This protocol is a simplified and condensed version of. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. 2. 2. 2K. Quant-iT PicoGreen dsDNA Assay Kit a. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). PicoGreen dsDNA quantitation reagents are. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. Thermo Fisher quant it picogreen dsdna quantification reagent kit Quant It Picogreen Dsdna Quantification Reagent Kit, supplied by Thermo Fisher, used in various techniques. The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. Table 2 summarizes results of the. P11495. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. Quantitation using Quant-iT Assay Kits. The assay is highly selective for double- stranded DNA over RNA, and in the. 3 Q33120 39. No. This kit contains. PicoGreen assay. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Picogreen assays are compatible with most microplate readers and fluorometers. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. Picogreen assays are compatible with most microplate readers and fluorometers. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. 2 to 100 ng) Assay Kit were used with a Qubit 3. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. Measure concentration with a high-sensitivity assay (e. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. PicoGreen Solution must be diluted fresh before each assay run. 2. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. no. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. No. 作成者 LatB Staff 01. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 4). • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. An aliquot (1. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. ” application on Lunatic and compared to Qubit and. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. 86 / 100 stars. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. 3. Sample fluorescence was. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Optimized for fluorescence microplate readers. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. 1 mL kit. 2. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Figure 2. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. Rapidly detect as little as 0. 10. 2. 1. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 8. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 适用于（设备）. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. DNA. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. • Suitable for use in 96- and 384-well plate formats. Katalognummer: P11496. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. PicoGreen dsDNA quantitation reagents are. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. 2. Determination of DNA Yield. 5), 200X PicoGreen. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. After a 5-minute. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. The normalized DNA was aliquoted into multiple tubes at 50µL volume. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Table 2 summarizes results of the. high-range. One microgram of DNA was sheared using an M220. For Research Use. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. Picogreen assays are compatible with most microplate readers and fluorometers. 99. 2. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Selectively detect dsDNA from a wide variety of sources, and even in presence of. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. 125 to 100 ng/mL. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. 0. Product Type. Q33120). The The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). The assay is linear over three orders of magnitude and has little sequence dependence. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). QuantiFluor® ONE dye sensitivity range is 0. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Optimized for fluorescence microplate readers. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. 2. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. Table 2 summarizes results of the. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Thermo Fisher quant it picogreen dsdna quantitation assay kit. Not for use in diagnostic procedures. (2012). 5mL. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 2 to 100 ng) Assay Kit were used with a Qubit 3. Catalog Number. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Got it. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Figure 8. 0 mL to 200 uL to fit a 96-well microplate format. Free dye. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 8 Measurement of growth factor content in. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. Picogreen assays are compatible with most microplate readers and fluorometers. Table 2 summarizes results of the. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. 10 x 100 µL. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. Bioz Stars score: 86/100, based on 1 PubMed citations. P7589. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Picogreen assays are compatible with most microplate readers and fluorometers. 15日开始对本试剂盒内标品（B组分）进行更新，由原来的小牛胸腺DNA升级为Lamda DNA，内部测试后者与Picogreen具更佳的结合性和荧光信号。后续统一按照新标品供货。Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). 7 Q33231 6 1,000 41. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. no. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. Bioz Stars score: 99/100, based on 1 PubMed citations. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Rapidly detect as little as 0. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. Fluorometric dsDNA Assay. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. The accuracy index is defined in Table 2. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 1 mL kit. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Description. Similar. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. n = 3. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. 99 to $1999. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Rapidly detect as little as 0. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. The Rapidly detect as little as 0. 5). Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. 9. The PicoGreen is sticking to the sides of the cuvette or assay plate. Volume of 20xTE = final volume/20;Application tip demonstrating that with Molecular Devices SpectraMax® microplate textbooks and the Quant-iT PicoGreen assay, users can reliably measure contents as low when 50 pg/mL of double-stranded DNA. • Specific for dsDNA in the presence of RNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2–100 ng, the fluorescence signal is linear with DNA. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Product Type. 5 Q32851 15. PicoGreen dsDNA quantitation reagents are. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 5. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Bioz Stars score: 86/100, based on 1 PubMed citations. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. no. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 製品番号（カタログ番号）： P7581. 005% Triton X-100, Tween-20, or Brij. Katalognummer: P11495. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 产品概述. Quantitation Range. Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). 86100 stars. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The PicoGreen reagent was discarded and the cells were rinsed with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) solution for 10 min. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. クイックオーダー. No. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Rapidly detect as little as 0. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). AccuBlue® RNA Quantitation Kit Details. High tolerance for contaminants. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. For duplicate-well droplet. It must be completely liquified before use by allowing it to come to room temperature. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. 2. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Briefly, 2 µL of sample was diluted in 498 µL TE. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. 50 pg to 2 µg. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). 9 Q33230 9 500 28. 2009;49(4):997–1002. Figure 2. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 04 corresponds to 2 μ g/mL dsDNA solution. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. Results: DNA concentration values were measured by the “DNA Mamm. Figure 1. ITSkabir). In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. assay. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. USA) and quantity was measured using Qubit 2. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Picogreen assays are compatible with most microplate readers and fluorometers. Three kits covering a wide range of dsDNA concentrations. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. Technical Support Customer Service. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Facebook. 5 µL of each extracted sample was used for Picogreen analysis. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. 5 µL of each extracted sample was used for Picogreen analysis. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. Picogreen assay kit (Invitrogen, P7589) a. Figure 8. Quant-iT PicoGreen dsDNA Reagent. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. Calibrator MPO. • Suitable for use in 96- and 384-well plate formats. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. 10pg/µl vs. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. 2. low-range. io |. 2 ng to 100 ng of DNA in the assay tube. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Quantitation Range. Quant-iT PicoGreen dsDNA Assay Kit. samples as well as standard in the assay itself. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2.